p53 Gene Mutations in Non-Small Cell Lung Cancer
from Thai Patients
SONGSAK PETMITR, Ph.D.* ,
PANTAP SUTINONT, M.D.**,
SIRIWAN MAKTHIENGTRONG,M.Sc.*,
PANATA MIGASENA, M.D., Ph.D., F.R.S.(T)*
Affiliation : * Department of Tropical Nutrition & Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok I 0400.
** Department of Pathology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok I 0700, Thailand.
AbstractParaffin embedded tissues from twenty-two Thai patients with non-small cell lung
cancer were studied for p53 gene mutations in exon 5 to 8 using polymerase chain reaction and
single-stranded conformation polymorphism (PCR-SSCP) followed by thermal cycle sequencing.
Results showed that point mutations in this region of p53 gene were present in 3 cases. One
harboured the base change from QAC to AAC at codon 281, changing amino acid from aspartic
acid to asparagine, whilst the other two cases were transversion of AAA (lysine) to Ai:A (threonine)
at codon 292. All subjects with p53 mutation had a past history of tobacco smoking.
Keywords : p53 Gene, Lung Cancer, Thai Patients
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