Effects of Different Biopsy Methods on the Development of Preimplantation Mouse Embryos, in vitro and in vivo : Implication for Preimplantation Genetic Diagnosis

PORNPIMON TANGCHAISIN, M.Sc.*, THA WEESAK TIRA W ATNAPONG, Ph.D.***, PRAMUAN VIRUTAMASEN, M.D.** KAMTHORN PRUKSANANONDA, M.D.**, VITHAYA YODYINGYUAD, Ph.D.****,

Affiliation : * Interdepartment of Physiology, * * Division of Reproductive Medicine, Department of Obstetrics & Gynecology, *** Division of Immunology, Faculty of Medicine, **** Department of Biology, Faculty of Science, Chulalongkom University, Bangkok I 03330, Thailand.

AbstractThe aim of this study was to investigate the efficacy of two different embryo biopsy techniques, direction aspartial zona dissection (PZD)-push, on subsequent in vitro and in vivo development of 8-cell stage mouse embryos. It was found that the rates of normal blastocyst formation and hatching blastocysts of direct aspiration, PZD-push, solution control and control embryos were not significantly different (80.8%, 81.6%, 84.5%, 86.7% and 71.9%, 72.3% and 74.6%) respectively. There was, however, a significant reduction in rate of complete hatching blastocysts (P

Keywords : Embryo Biopsy, Preimplantation Genetic Diagnosis (PGD), Development in vitro and in vivo

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