Identification of Sex Chromosome by Fluorescence in situ Hybridization

SASAKORN SARASOPHONA, M.Sc. *, KOTCHAKORN PROMSAWAT, Cert.M.L.T.*, BUDSABA RERKAMNUAYCHOK, D.M.Sc.*

Affiliation : * Human Genetics Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University. Bangkok 10400, Thailand.

AbstractFluorescence in situ hybridization (FISH) is the complicated and very effective technique to determine the origin of chromosome material that cannot be identified by conventional banding techniques. Also determining the hidden sex chromosome and the percentage of mosaicism. Five peripheral blood and. one cord blood sample were used to perform centromeric X and Y chromo- some-specific DNA probe to determine the sex chromosome. Comparing the percentage of mosai- cism between conventional cytogenetic technique and FISH technique, we found a different ratio in mosaicism. That is because the molecular cytogenetic study was the evaluation of chromosome identification in both dividing (metaphase) and non-dividing cells (interphase nuclei).

Keywords : FISH, Sex Chromosome

All Articles Download

Information

Related Links