Comparison of DNA Extraction From Blood Stain and Decomposed Muscle in STR Polymorphism Analysis
BUDSABA RERKAMNUAYCHOKE, D.M.Sc.*,
UBONRAT JOMSAWAT,B.Sc.*,
NANTAPRON PATTANASAK, R.N.***,
WASUN CHANTRATITA, Ph.D.**,
JANPEN THANAKITGOSATE, B.Sc. *,
PORNTIP ROJANASUNAN, M.D.****
Affiliation : *Human Genetics Unit,
**Virology and Molecular Microbiology Unit,
***Out-Patient Department,
****Forensic Pathology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
AbstractForensic samples that are often degraded and limited in quality cause DNA typing analy-
sis by conventional methods unsuitable. We performed a single tube-multiplex PCR on 9 STR loci
(D3Sl358, vWA, FGA, THOl, TPOX, CSFIPO, D5S818, DI3S317, and D7S820) and the X-Y
homologous gene amelogenin of DNA extracted from six week postmortem blood stain and
decomposed muscle by using QIAGEN QIAamp blood or tissue procedure. An automated genetic
analyzer based on fluorescent dye technology was used to detect STR allele patterns. The DNA
profile of blood stain sample obtained a complete and unambiguous pattern, whereas, that of muscle
DNA extracted from QIAamp tissue and Chelex plus QIAamp blood protocols showed detected
STR alleles for 70 per cent and 50 per cent of all tested alleles, respectively. The degraded muscle
DNA could not yield amplified products of large size STR alleles; CSFIPO, Dl3S317 and D7S820.
However, the analysis which relied upon the PCR-based STR polymorphism analysis and automated
genetic analyzer system offers an ideal strategy for forensic identification.
Keywords : DNA, Decomposed Muscle
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