Fluorescence in situ Hybridization : A Rapid Analysis to Verify Chromosome Aberrations
CHINTANA TOCHAROENTANAPHOL, Dr.sc.hum. , RACHANEE PARINAYOK, M.Sc. ,
NAPASSAWAN PUNTHUWATTANA,B.Sc.**,
DAOROONG KANGWANPONG,Dr.rer.nat.***
Affiliation : * Department of Pathology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10110.
** Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400,
*** Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50202. Thailand.
AbstractWe applied fluorescence in situ hybridization (FISH) to assess the presence of structural
rearrangement and numerical chromosome aberrations in both metaphase chromosome and inter-
phase nuclei. For this purpose, the biotinylated repetitive alpha-satellite DNA probes for chro-
mosome I, 18 and 8 (pUCI.77, LI.84 and pJM128) were used to identify tetraploid mosaicism,
ring chromosome 18 and trisomy 8 mosaicism for pre-, post-natal and tumor diagnosis res-
pectively. Utilizing this approach, we showed the usefulness of FISH for routine clinical cyto-
genetics in addition to chromosome banding techniques. The chromosome aberrations with
unknown or unclear origin, detected by chromosome analysis, could be confirmed accurately
and rapidly.
Keywords : Fluorescence in situ Hybridization (FISH), Structural Chromosome Rearrangement, Numerical Chromosome Aberration, Mosaicism
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