BCRIABL Rearrangement in Philadelphia Chromosome Negative CML Patients

BUDSABA RERKAMNUAYCHOKE, D.M.Sc.*, NAPATSA WAN MANEESRI, B.Sc. *, NANTAPRON PATTANASAK, R.N.* UBONRAT JOMSAWAT, B.Sc.*, AKSARA APILUGSANACHIT, B.Sc.*,

Affiliation : * Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400 Thailand.

AbstractReverse transcription-polymerase chain reaction (RT-PCR) was used to study 34 patients with chronic myelogenous leukemia (CML) associated with negative Philadelphia (Ph) chromo- some. This report showed evidence of a chimeric BCR/ABL transcript in 18 (52.9%) and 28 (82.4%) cases by first PCR and seminested PCR, respectively. In these BCR/ABL transcript positive cases, the incidence of BCR exon3/ABL exon2 (B3A2) and BCR exon 2/ABL exon2 rearrangement was 25 (89.3%) and 3 (10.7%) cases, respectively. The other 6 Ph negative patients showed no evi- dence of reciprocal translocation of BCR to chromosome 9. This data demonstrates that seminested PCR is sufficiently sensitive to detect BCR/ABL fusion transcript in Ph chromosome negative CML patients.

Keywords : BCR/ABL, Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Chronic Myelogenous Leukemia (CML)

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