Paternity Testing by PCR-Based STR Analysis
BUDSABA RERKAMNUAYCHOKE, D.M.Sc.*, UBONRAT JOMSAWAT,B.Sc.*, PORNTIP ROJANASUNAN, M.D.*** WASUN CHANTRATITA, Ph.D.**, JANPEN THANAKITGOSATE, B.Sc. *,
Affiliation : *Human Genetics Unit, **Virology and Molecular Microbiology Unit, ***Forensic Pathology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University. Bangkok 10400, Thailand.
We present application of polymerase chain reaction (PCR) - based short tandem repeat (STR) system for use in paternity testing. The process involves a single tube multiplex PCR of 9 STR loci on different chromosomes, in conjunction with Amelogenin sex test and internal size standards, followed by using an automated DNA sequencer to detect amplified products. The results showed that this system provided unambiguously reliable results. In addition, the method is useful for routine use in that it is robust and reproducible and provides a reliable means of pater- nity testing.
Keywords : Paternity Testing, PCR, STR
