Forensic Identification by DNA Fingerprinting and
Mitochondrial DNA Typing
BUDSABA RERKAMNUAYCHOKE, D.M.Sc.*,
WASUN CHANTRATITA, Ph.D.**,
UBONRAT JOMSAWAT, B.Sc.*,
JANPEN THANAKITGOSATE, B.Sc.*,
PORNTIP ROJANASUNAN, M.D.***
Affiliation : *Human Genetics Unit,
**Virology and Molecular Microbiology Unit,
***Forensic Pathology Unit, Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University.
Bangkok 10400, Thailand.
AbstractShort tandem repeats (STRs), that represent an important source of highly polymorphic
markers in human genome, and mitochondrial DNA (mtDNA) typing, that its sequences were
conserved within the same maternal lineage, facilitated by use of the polymerase chain reaction
(PCR) provide a powerful tool for forensic identification. We report the analysis of 9 STR loci
and mtDNA typing of a muscle biopsied sample with 2 months postmortem by comparison with
the genotype of the relative. The DNA profile showed common alleles with that of the relative
but only 12 from 20 alleles (60%) were identifiable. Then, we performed mt DNA sequencing
of the hypervariable region I (HV I) and obtained 100 per cent homology with that of the relative.
In conclusion, personal identification can be performed precisely by the data of DNA profile and
mtDNA typing compared to the genotype of the relative.
Keywords : Forensic Identification, DNA Fingerprinting, mtDNA
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