Semiquantitative Determination of Urinary Glucose :Comparison of Home-Made Strip and Routine Tests

RAUNGRAIW AN KOONAKOSIT, M.Sc. *

Affiliation : *Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400. Thailand.

AbstractGlucose oxidase paper strips for semiquantitive determination of glucose in urine are com- mercially available but details of their preparation are not published. The purpose of this study was to produce such strips, using a coupled enzyme glucose oxidase-peroxidase reaction which would yield purple color with ortho-tolidine, and safranin upon dipping into urine containing glucose. In this present study, without any special equipment, special humidity and temperature control, the new reagent strip named R-strip could be successfully prepared in the atmospheric conditions of Thailand. R-strips were evaluated against random urine added with various amounts of glucose in comparison with a commercial strip (T-strip), Benedict's test, and a commercial tablet (C-tablet), routinely used in laboratories. The developed strips were found to be as specific as T-strip and more sensitive than other tests.

Keywords : Semiquantitative Test Strip, Urinary Glucose, Glucose Oxidase Test

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