Modification of the Acetylcholinesterase (AchE) Staining Method in Hirschsprung's Disease

VILAI CHENTANEZ, M.D., Ph.D*, SOOTTIPORN CHITTMITTRAPAP, M.D.**, VIRA KASANTIKUL, M.D.***

Affiliation : *Department of Anatomy, **Department of Surgery, *** Department of Pathology, Faculty of Medicine, Chulalongkorn University. Bangkok I 0330 Thailand.

AbstractDemonstration of the increasing activity of acetylcholinesterase (AChE) in a segment of the colon has proved to be the most accurate diagnostic tool to diagnose Hirschsprung · s disease. Two methods of histochemical assessment were tried to establish the most appropriate and effective method for this study within the limitation of available equipment. Lake· s method was chosen and was modified as the standard histochemical examination.

Keywords : Acetylcholiesterase Staining, Rectal Suction Biopsy, Hirschsprung's Disease

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