Investigation of Sex Chromosome Abnormalities in Teratozoospermia of Infertile Men using Fluorescence in Situ Hybridization

ANEK ARIBARG, F.R.C.O.G. *, JIRAPORN NGEAMVIJAWAT, B.Sc.*, YENCHIT CHANPRASIT, B.Sc.*

Affiliation : * Andrology Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University. Bangkok 10330, Thailand.

AbstractFluorescence in situ hybridization is a fast and efficient method of investigating chro- mosomal abnormalities in human s~fi!latp_zQ_a. In this study, we have established the frequency of sex chromosome disomy (XX, XY and YY) in teratozoospermia (98-1 OOlk abnormal mor- phology) of infertile men compared with normospermic men using double-probe FISH pro- cedures. A total of 40000 sperms were scored in each group of semen for chromosome X and Y with overall hybridization efficiency of 97.00 per cent and 98.02 per cent in infertile men and normal men, respectively. It was found that the frequency of disomy XX, XY and YY was sig- nificantly higher in infertile men compared with normal men (P<0.05). It is concluded that the infertile men showed increased frequencies of sex chromomsomal abnormalities.

Keywords : Sex Chromosome Abnormalities, Male Infertility

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