Development of Coagglutination Reagents for Serolo-
gical Grouping of Streptococci
ORRAWADEE HANVIVATVONG,M.Sc.*,
CHADAPORN CHUTICHOP, B.Sc. **,
SUMANEE SIRILERTPANA, M.Sc.**,
SURANAN TIRA W ATNAPONG, M.Sc. *
Affiliation : * Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330,
** Department of Microbiology, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok 10330,
Thailand.
AbstractCoagglutination reagents for the rapid serological grouping of groups A, B, C, F and G
Streptococcus have been developed. Antisera to groups A, B, C, F and G Streptococcus were raised
in rabbits. After absorption with cross-reacting antigens, the specific antibodies were coated on
Staphylococcus protein-A and used as group-specific coagglutination reagents. The sensitivity of
the reagents for groups A, C and G Streptococcus was 100 per cent and the specificity was I 00,
100, and 98.77 per cent, respectively. The sensitivity and specificity of these reagents were con-
sistent up to 12 months, although specificity declined with longer storage. The in-house coagglu-
tination reagents for groups A, C and G streptococcus were also tested in comparison with the
commercially available Streptococcus Phadebact® test and yielded almost identical results. Sensi-
tivity of the in-house of group B Streptococcus reagent was low, while the group F reagent gave
a high incidence of false positive reaction.
Keywords : Coagglutination, Streptococci
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