In Vitro Susceptibility Testing of Levofloxacin and
Ofloxacin by Microtiter Plate Alamar Blue Against
Multidrug and Non Multidrug Resistant Mycobacte-
rium tuberculosis in Thailandt
ROONGNAPA PRACHARKTAM, B.Sc., M.Sc.*,
KANUNG ANGKANANUKOOL, B.Ed. (Biology)*,
ASDA VIBHAGOOL, M.D.**
Affiliation : ** Division of Infectious Disease, Department of Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol
University, Bangkok 10400, Thailand.
t This study was supported by Daiichi Pharmaceutical (Thailand) Ltd.
AbstractAntituberculous drugs for current therapy of multidrugs resistant tuberculosis (MDR-TB)
are limited. The in vitro susceptibility of Mycobacterium tuberculosis (MTB) as well as MDR-
TB with other antibiotic drugs were determined in order to find alternative drugs for the treat-
ment of MDR-TB. Forty-seven MDR-TB and 62 MTB of clinical isolates were tested against
levofloxacin and ofloxacin. The MDR-TB at the MIC90 of levofloxacin and ofloxcain were 1 J.lg/
ml and 2 J.lg/ml, respectively. Of these MTB, the MIC90 of both drugs were 0.5 f.lg/ml and 1
f.lg/ml, respectively. It seemed that levofloxacin MICs of both MDR-TB and MTB were one
dilution less than ofloxcin. The promising activity of ofloxacin and levofloxacin against MDR-
TB and MTB suggest that both drugs could be used as second-line drugs for MDR-TB or as a
good alternative antituberculous drug for patients who have intolerance to the first line drug.
Keywords : Mycobacterium tuberculosis, Tuberculosis, Levofloxacin, Ofloxacin, Alamar Blue
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