Comparison of Embryonic Development in Cleavage Stage Mouse Embryo Biopsy Between Acid Tyrode's Solution and Laser Assisted Techniques
PHAKPHUM PHOPHONG, M.D., M.Sc.*, ALPESH DOSHI, M.Sc. **, JOYCE C HARPER, Ph.D.***
Affiliation : * Department of Obstetrics and Gynaecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok I 0700, Thailand. ** The Assisted Conception Unit, University College Hospital, the United Kingdom. *** Department of Obstetrics and Gynaecology, University College London, the United Kingdom.
The study was carried out to detennine the effectiveness and safety of the infrared 1.48 ~m laser in cleavage stage mouse embryo biopsy, compared to the conventional acid Tyrode's solution. One hundred and thirty cryopreserved cleavage stage mouse embryos were included in the study. Fifty embryos were biopsied by acid Tyrode's solution. Forty-seven embryos were biopsied by the infrared 1.48 ~m laser. Thirty-three embryos were incubated without biopsy as the control group. Thirteen of 50 embryos in the acid Tyrode's group and 16 of 47 in the laser assisted group became cavitating morulae on day 4, meanwhile 23 of 33 in the control group reached this stage. The blastocyst fonnation of acid Tyrode's, laser assisted and control group were 94.0, 97.8 and 100.0 per cent, respectively. The hatching rate of acid Tyrode's solution, laser assisted and control group were 78.7, 84.7 and 63.6 per cent, respectively. No significant diffe- rence in blastocyst fonnation and hatching rate was found. The percentage of grade I blastocysts in control group (96.9%) was significantly higher than those in acid Tyrode's solution (68.0%) and the laser assisted group (76.0% ). There was no significant difference in the percentage of grade 1 blastocysts between the acid Tyrode's solution and the laser assisted group. In conclusion, the infrared 1.48 ~m wavelength laser may be an alternative to acid Tyrode's solution in embryo biopsy.
Keywords : Key word Laser, Acid Tyrode's Solution, Embryo Biopsy, Preimplantation Genetic Diagnosis, Blastocyst
