Evaluation of katG Codon 315 Mutations Among Iso niazid Sensitive and Resistant Mycobacterium tuber culosis Isolates from Thailand

PANKORN IMWIDTHA YA, M.D., Dr.med.*, KLAUS MIESKES, M.Sc.**, SOMSAK RIENTHONG, M.Sc. ***

Affiliation : * Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. ** Department of Infectious Diseases and Tropical Medicine, Klinikum Innenstadt, University of Munich, Leopold strasse 5, D-80802 Munich, Germany. *** Tuberculosis Division, Department of Communicable Diseases Control, MoPH, Bangkok 10120, Thailand.

AbstractNine isoniazid (INH)-susceptible and 11 INH-resistant Mycobacterium tuberculosis clini cal isolates were analyzed for katG codon 315 mutations by polymerase chain reaction (PCR) assay using primers MYC-32 and MYC-33, followed by restriction fragment length polymorphism. After Acil digestion of PCR products, all 9 INH-susceptible isolates and 5 out of 11 ( 45%) INH-resistant isolates showed 0.12 kb band, which was previously reported to indicate wild type, whereas, 6 of 11 (55%) INH-resistant isolates lacked this band.

Keywords : M. tuberculosis, katG Codon, INH Susceptibility

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