Isomeric Separation of Methamphetamine by HPLC Chiral Column

VEERA VAN LEKSKULCHAI, Ph.D.*

Affiliation : * Department of Pathology, Faculty of Medicine, Srinakharinwirot University, Bangkok 10300, Thailand.

AbstractMethamphetamine and its active metabolite, amphetamine, are optically active compounds which, based upon synthetic routes, can be found in two forms; pure d-form and racemic mixture. Analysis of their isomers can help to identify which precursor is currently spreading widely in a given region. Since there are many drugs that can be metabolized to amphetamine/methampheta- mine, isomeric separation can be a useful tool for evaluation of these drugs, as well. Indirect method by using N-trifluoroacetyl- 1 -prolyl chloride (1 -TPC) was found to have limited accuracy due to the contribution effect. In this presentation a direct method using HPLC Chirex chiral column 3022 was studied. Although the method gave no base-line separation of two different isomer peaks, it gave good sensitivity, reliability, and linearity. No contribution effect was found in the method presented. It also gave excellent correlation with the 1-TPC method.

Keywords : Methamphetamine, Isomeric Separation, Chiral Column

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