Effects of Sodium Butyrate and Acidic Fibroblast Growth Factor on TDC32300 Cultured Cells

PANEEYA PIENVICHIT, M.D.*, TAMAKO A. KONKIN, B.A.**, RONALD A. FARIS, Ph.D.**

Affiliation : * Division of Pediatric Gastroenterology, Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand. ** Division of Pediatric Hematology and Oncology, Department of Pediatric, Hasbro Children's Hospital, Brown University, Providence, Rhode Island, USA.

AbstractBackground : Many studies have demonstrated that transition duct cells (TDC) are faculta tive liver stem cells. Our laboratory established TDC32300 cell lines with hepatic progenitor markers. The authors proposed that cell culture using sodium butyrate (NaBut) and acidic fibroblast growth factors (aFGF) may support the differentiation of TDC32300 cells along the hepatic lineage. Methods : TDC32300 cells were cultured in four different conditions 1) STON media alone; 2) STON with NaBut in 3 different concentrations, 1 mM, 3.75 mM and 5 mM; 3) STON with aFGF; and 4) STON with aFGF and dexamethasone. After day 5, the cultured cells were fixed and stained with monoclonal antibodies to rat liver antigens and anti-proliferating nuclear antigen (PCNA).
Results : Proliferation of TDC32300 cells cultured in the high concentration of NaBut (3.75 and 5 mM) was inhibited. This phenomenon was confirmed by the reduction in cell number and decrease in PCNA expression. Irrespective of the concentration, NaBut did not alter the phenotype of the TDC32300 cultured cells. aFGF with or without dexamethasone also did not alter the pheno typic characteristic of TDC32300 cells.
Conclusion : TDC32300 cells may not be the hepatic progenitors or that their differentia tion may require other culture conditions.

Keywords : Sodium Butyrate, Acidic Fibroblast Growth Factor, Transitional Duct Cell, Facultative Liver Stem Cell

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