The Effect of Growth Hormone on the Development of In Vitro Matured Unstimulated Human Oocytes
AREEPAN SOPHONSRITSUK, M.D.*, SOMCHAI SUWA JANAKORN, B.Sc., M.D.*, KAMTHORN PRUKSANANONDA, M.D.*, WISAN SEREEPAPONG, M.D.*, VICHUDA AHNONKITPANIT, M.Sc.*, DOENTIP CHOMPURAT, B.Sc.*, PRANEE NUMCHAISRIKA, B.Sc. *, WISUT BOONKASEMSANTI, M.D.*, PRAMUAN VIRUTAMASEN, M.D.*
Affiliation : *Department of Obstetrics & Gyn!lecology, F!lculty of Medicine, Chulalongkom University, Bangkok 10330, Thailand.
Objective : To investigate the effect of growth hormone on the development of in vitro matured unstimulated human oocytes.
Design : Randomized controlled study
Setting : Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkom university
Materials and Methods : 108 germinal vesicle-stage oocytes were retrieved from 47 patients undergoing gynecologic surgery. They were aspirated either during gynecologic surgery or from excised ovaries. The oocytes were then cultured in vitro with or without growth hormone (1,000 ng/ ml) in medium199 supplemented with sodium pyruvate, FSH, LH, antibiotic and synthetic serum. Incubation was done at 37 degree C with 5 per cent C02 in air and nuclear stage was assessed after 18, 42, 66 and 90 h of incubation.
Main outcome measure : Attainment of metaphase II and GVBD
Results : After in vitro culture, there were no significant differences in maturation and GVBD rate. 27 of 52 (51.9%) oocytes (GV) in growth hormone group matured to metaphase II compared with 25 of 53 (47.2%) GV in control group. GVBD rate for germinal vesicle-stage in growth hormone group was 76.9 per cent compared with 79.2 per cent in control group.
Conclusion : Culture of immature oocytes in vitro with growth hormone results in similar maturation rate as that without GH.
Keywords : Growth Hormone, In Vitro Maturation, Immature Oocyte
