Epstein-Barr Virus-Associated Non-Hodgkin's Lymphoma
of B-cell Origin, Hodgkin's Disease, Acute Leukemia, and
Systemic Lupus Erythematosus : A Serologic and Mole
cular Analysis
WINYOU MITARNUN,M.D.*, V ANNARAT SAECHAN, M.Sc. *,
JINTANA PRADUTKANCHANA, M.Sc.*, SUPAPORN SUWIWAT, M.Sc.*,
SATOMI TAKAO, M.Sc.**, TAKAFUMI ISHIDA, D.Sc.**
Affiliation : *Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla 90110, Thailand.
** Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
AbstractParallel studies of (a) patients with Epstein-Barr virus (EBV)-associated peripheral T-cell
proliferative disease/lymphomas and (b) a group of patients with a prolonged fever from other causes
were conducted at Songklanagarind University Hospital from 1997 through 2000. (Reports on EBV
associated peripheral T-cell and NK-cell proliferative disease/lymphomas have been published else
where) In this study, the authors identified 58 patients; 14 were non-Hodgkin's lymphoma of B-cell
origin (NHL-B), 8 were Hodgkin's disease, 6 were acute leukemia, 9 were systemic lupus erythema
tosus (SLE), and 21 were patients with other diseases. Serologic tests for the EBV infection, the study
of EBV genome in circulating non-T-cells (CD3-cells) and T-cells (CD3+ cells), and the EBV-RNA
study in the tumor cells were performed. EBV internal repeat-1 region (IR-1) in peripheral blood CD3+
cells was detected in 10 of 14 patients (71.5%) with NHL-B, 3 of 8 patients (37.5%) with Hodgkin's
disease, 1 of 6 patients (16.7%) with acute leukemia, 4 of 9 patients (44.5%) with SLE, and was not
detected in any of the 21 patients with other diseases. Anti-viral capsid antigen-IgG was significantly
elevated in hematologic malignancy patients with EBV IR-1 genome in the peripheral blood CD3+
cells when compared to hematologic malignancy patients with a negative result, whereas there was
no significant difference in anti-EBV nuclear antigen among these two groups. EBV-RNA expres
sion in tumor cells by in situ hybridization was detected in 4 of 13 patients (31%) with NHL-B (all
showed EBV IR-1 genome in peripheral blood CD3+ cells), and 3 of 5 patients (60%) with Hodgkin's
disease (only two showed EBV IR-1 genome in peripheral blood CD3+ cells). These data support
the theory that chronic EBV infection is often found in association with cases of NHL-B, Hodgkin's
disease, acute leukemia, and SLE.
Keywords : Epstein-Barr Virus, Non-Hodgkin's Lymphoma, Hodgkin's Disease, Acute Leukemia,
Systemic Lupus Erythematosus, CD3+ Cell
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