Antispasmodic Effects of Curcuminoids on Isolated Guinea Pig Ileum and Rat Uterus

CHANDHANEE IITHIPANICHPONG, M8c*, NU8IRI RUANGRUNG81, PhD**, WA NDEE KEM8RI, B8c*, ANUGOOL 8AWA8DIPANICH,M8c*

Affiliation : • Department of Pharmacology, Faculty of Medicine, •• Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.

AbstractCurcuminoids, a yellow constituent isolated from Curcuma longa Linn. rhizomes was studied for its antispasmodic activity in isolated guinea-pig ileum and rat uterus. Curcuminoids at the con centration of 12 IJ.g/ml significantly inhibited the ileum pre-contracted with acetylcholine (ACh) 5 x I0-7 M .and histamine 5 x I0-7 M. (Force of contraction was 62.84 ± 4.66% and 75.60 ± 4.66% respectively) and the effects were prominently observed when the concentration of curcuminoids was increased to 36 IJ.g/ml. (Force of contraction ~as 44;93 ± 4.33% and 42.79 ± 1.98%) In potassium depolarizing Tyrode solution, ·curcuminoids 41J.g/ml and 20 IJ.g/rtll reduced the contraction induced by calcium chlo ride (CaCI ) 1.8 mM. (The contraction was 63.31 ± 1.80% and 36.87 ± 3.25%) In rat uterus smooth 2muscle preparation, curcuminoids 8 IJ.g/ml and 16 IJ.g/ml significantly reduced force and frequency of contraction induced by oxytocin I x I0-2 IU/ml. Curcuminoids 8 IJ.g/ml produced 54.68 ± 3.34 per cent force of contraction and 79.09 ± 2.29 per cent frequency of contraction. Curcuminoids 161J.g/ml caused more relaxation of rat uterus smooth muscle. (Force of contraction. was 43.38 ± 3.56%, frequency of contraction was 49.96 ± 5.20%). Curcuminoids 8 and 161J.g/ml significantly reduced force of contrac tion induced by KCI 50 mM. (Force of contraction was 54.10 ± 4.92% and 36.60 ± 2.99%). The results obtained from this study concluded that curcuminoids produced a smooth muscle. re~axation effect on isolated guinea-pig ileum and rat uterus by receptor-dependent and independent mechanism:

Keywords : Curcuminoids, Antispasmodic, Turmeric

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