Serological analysis of Human Leukocyte Antigens-A and -B Antigens in Thai patients with Nasopharyngeal Carci noma
NA TTIYA PIMTA NOTHAI, MD, PhD*, ORAT Al KANGWANSHIRATADA, MSc*, PREEY AC HIT CHAROENWONGSE, MD*
Affiliation : *Immunology Unit, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
Objective : To study the distribution of human leukocyte antigens (HLA) -A and -B antigens by standard microlymphocytotoxicity assay in Thai nasopharyngeal carcinoma (NPC) patients com pared to normal controls in order to identify the alleles associated with NPC in Thailand
Design : Retrospective-Analytical study
Subjects : Fifty-three unrelated Thai patients with histologically confirmed NPC diagnosed at King Chulalongkom Memorial Hospital and 70 healthy unrelated Thai individuals served as con trols
Method : Lymphocyte separation and HLA typing were performed from freshly drawn blood by standard microlymphocytotoxicity assay. The significance of differences between the two groups was analyzed by the chi-square test
Results : HLA-A2 was observed at a greater frequency in patients being found in 31/53 (58%) = NPC patients compared to 27170 (38%) controls (p 0.02). An increase in HLA-B46 was also demon strated. HLA-B46 was present in 16/53 (30%) NPC patients but was observed in 10170 (14%) in con = trols (p 0.03).
Conclusions : This study reported two susceptible, HLA-A2 and HLA-B46 antigens, for NPC in a Thai population.
Keywords : Nasopharyngeal Carcinoma, HLA-A, HLA-B
