Distribution of Human Leukocyte Antigens-E Alleles • Ill Thailand

INGORN KIMKONG, BSc*, APIW AT MUTIRANGURA, MD, PhD**, NA TTIY A PIMTA NOTHAI, MD, PhD***

Affiliation : *Inter-Department of Medical Microbiology, Graduate School, **Department of Anatomy, Faculty of Medicine, *** Department of Microbiology, Faculty of Medicine Chulalongkom University, Bangkok 10330, Thailand.

AbstractThe aim of this study was to investigate the distribution of human leukocyte antigens (HLA) -E alleles in Thailand. HLA-E alleles were assigned by using a polymerlase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) method and direct sequencing in 200 healthy individuals. They comprised 100 Thai, 50 Chinese and 50 Thai-Chinese. From the results, three alleles of HLA-E could be detected in these populations. The E*OlOl was the most common allele in Thai and Thai Chinese with allelic frequencies of 42.5 per cent and 38 per cent, respectively. The other HLA-E allele frequencies of Thai origin were 33 per cent for E*Ol031 and 24.5 per cent for E*Ol032, respectively. Among Thai-Chinese, the allele frequencies of HLA-E were 31 per cent for E*Ol031 and E*Ol032, respectively. Whereas, the E*Ol031 was the predominant allele in Chinese origin with a frequency of 39 per cent, followed by E*OlOl and E*Ol032 with 32 per cent and 29 per cent, respectively. No E*Ol033, E*Ol02 and E*Ol04 could be detected in all individuals. When comparing the distribution of HLA-E alleles between each of the populations (Thai vs Chinese, Thai vs Thai-Chinese and Chinese vs Thai-Chinese), no significant difference could be found among these populations. In addition, there was no significant difference of the distribution of HLA-E alleles between the study populations and other populations from Asian countries, reported previously. However, there were significant diffe = rences between the populations (Thai, Chinese and Thai-Chinese) and Danish (X2 = 15.64, p 0.0004; X2 = 24.58, p = 0.0000046; X2 = 14.69, p = 0.00065, respectively).

Keywords : HLA-E, Alleles, PCR-SSOP, Direct Sequencing

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