Submit manuscript

Special Article

Clinical Application of Forensic DNA Analysis: A Literature Review

Affiliation : Chairat Manasatienkij MD*, Dr Med, Chatchawin Ra-ngabpai MD*

* Department of Forensic Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand Polymorphic short tandem repeat (STR) analysis after polymerase chain reaction amplification is presently the most important analytical method of forensic DNA laboratory. Beside the forensic-purpose application, STR analysis is also necessary for chimerism testing after bone marrow or allogeneic stem cell transplantation. To increase success rate of the treatment and avoid adverse effect of the transplantation procedure, serial chimerism study has to be closely monitored. Many analytical techniques have been therefore developed to improve the accuracy and sensitivity. STR-PCR is currently the most commonly used analytical method for chimerism testing. Sensitivity of the method is about 3% of the minor DNA in a specimen. The method consumes a short period of time and is convenient due to availability of the commercial kits. High discriminative ability and well-validated interpretation procedure are the attractive characteristics of the method.

Keywords : Chimerism testing, Forensic DNA analysis, Bone marrow, Allogeneic stem cell transplantation, STR analysis


All Articles Download


INFORMATION

Contact info

JOURNAL OF THE MEDICAL ASSOCIATION OF THAILAND
4th Floor, Royal Golden Jubilee Building,
2 Soi Soonvijai, New Petchburi road,
Bangkok 10310, Thailand.
Phone: 0-2716-6102, 0-2716-6962
Fax: 0-2314-6305
Email: editor@jmatonline.com

JMed Assoc Thai
MEDICAL ASSOCIATION OF THAILAND
ISSN: 0125-2208 (Print),
ISSN: 2408-1981 (Online)
The content of this site is intended for health professionals.

Submissions

» Online Submissions » Author Guidelines » Copyright Notice » Privacy Statement

Other

» Journal Sponsorship » Site Map » About this Publishing System

© MEDICAL ASSOCIATION OF THAILAND. All Rights Reserved. The content of this site is intended for health professionals.