Mouse Blastocyst Vitrification Compared with the
Conventional Slow-Freezing Method
WA NWISA THARNPRISARN, MD*, SOMCHAI SUWAJANAKORN, MD*,
WISAN SEREEPAPONG, MD*, KUMTHORN PRUKSANANONDA, MD*,
WISUT BOO NY AKASEMSANTI, MD*, PRAMUAN VIRUTAMASEN, MD*,
VICHUDA AHNONKITPANIT, MSc*, DOENTIP CHOMPURAT, BSc*,
PRANEE NUMCHAISRIKA, BSc*
Affiliation : * Division of Reproductive Medicine. Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn
University, Bangkok 10330, Thailand.
AbstractTo compare mouse blastocyst survival after cryopreservation with vitrification and the slow
freezing method, one-hundred and forty-eight in vitro mouse blastocysts were randomly frozen by the
two methods: vitrification and conventional slow-freezing. After being thawed, the blastocysts were
assessed for survival and hatching rate. The survival rates of blastocysts cryopreserved by vitrifica
=
tion and slow-freezing were 68.33 and 65.52 per cent (p 0.89), whereas hatching rates were 51.22 and
=
44.74 per cent, respectively (p 0.64).Therefore, vitrification of blastocyst-stage-embryos may be a
useful, economic method for freezing the excess blastocysts in some centers where blastocysts are
routinely transferred.
Keywords : Mouse Blastocyst, Cryopreservation, Vitrification, Conventional, Slow-Freezing Method
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