Preliminary Study on Somatic Cell Nuclear Transfer in
Rabbits in Thailand
MONGKOL TECHAKUMPHU, DVM, Doctorat de 3 e cycle*, PRANEE NUMCHAISRIKA, BSc**,
SOMCHAI SUWAJANAKORN, MD**, KAMTORN PRUKSANANONDA, MD**,
WISUT BOONKASEMSANTI, MD**, VICHUDA AHNONKITPANIT, MSc**,
PRAMUAN VIRUTAMASEN, MD**
Affiliation : * Department of Obstetrics and Gynaecology an Reproduction, Faculty of Veterinary Science,
** Reproductive Medicine Unit, Department of Obstetrics and Gynaecology, Faculty of Medicine, Chulalongkom
University, Bangkok 10330, Thailand.
AbstractThe objective of the study was to develop the somatic nuclear transfer technique by using
rabbits as the model. The oocyte recipients aged 16 h post coitus were collected surgically from 20
superovulated rabbit doe with 28 and 40 mg Follicle Stimulating Hormone (FSH) after mating with
a vasectomized male. The metaphase II plate and I" polar body of oocyte was later aspirated by
enucleated micropipette under an inverted microscope. A single donor cell; cumulus cell or cultured
or frozen fibroblast cell from passage I to 9 were transferred to enucleated oocyte and fused with
triple DC pulses, 3.2 kv, 20 jls. The fused embryos were cultivated in TCM I99 NaHC0 + 10 percent fetal calf serum (FCS) for 4 days. The cleavage rate (2-cell stage) was 37.2 per cent (32/86)
from eight experiments, and I8.8 per cent (6/32) developed to the early morula stage. This study also
indicated that the enucleation pipette and the somatic cell type influenced the success.
Keywords : Rabbit, Fibroblast Cells, Cumulus Cells, Nuclear Transfer
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