Collection and Processing of Umbilical Cord Blood for
Cryopreservation
YAOWALAK U-PRATYA, MS*, SIRIKW AN BOONMOH, BSc*,
ORAT HAI PROMSUWICHA, BSc*, CHANYA THEERAPITAYANON, MS*,
LAKSAMI KALANCHAI, BSc**, VIRUCH CHANJERBOON, MS**,
KORAKOT SIRIMAI, MD***, SANAN VISUTHISAKCHAI, MD*,
SASITORN BEJRACHANDRA, MD**, SURAPOL ISSARAGRISIL, MD*
Affiliation : * Division of Hematology, Department of Medicine,
** Department of Transfusion Medicine,
*** Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok
10700, Thailand.
AbstractUmbilical cord blood (UCB) is being increasingly used as an alternative source of hemato
poietic stem cells for allogeneic bone marrow transplantation. UCB transplantation has been success
fully used to treat a variety of genetic, hematological, and oncological disorders in children and adults.
The objectives of this study was to establish a closed-system technique for UCB collection and buffy
coat separation by Optipress I device. Thirty-four UCB were collected by triple-bag system from preg
nant mothers whose fetuses were not affected by thalassemic diseases after prenatal diagnosis. The
mean volumn of UCB collection were 120±5 ml (range 65-180 ml). Total WBC, CD34+ cells, the
progenitor cell erythroid burst-forming unit (BFU-E) and granulocyte-macrophage colony-forming unit
(CFU-GM) in the UCB units were (9.36 ± 0.84) x 108 (3.61 ± 0.52) x 106 (9.12 ± 1.60) x 105 and
, , ,(5.32 ± 1.23) x 105 respectively. Good correlation between the nucleated cell and net cord blood volume
,could be demonstrated (p < 0.0001). The correlation between CD34+ cells and the following para
=meters: nucleated cell, BFU-E or CFU-GM were also demonstrated (p 0.001, 0.0105 or 0.0001, res
pectively). Buffy coat was subsequently separated from 18 UCB units by Optipress I device. 70 ± 3 ml
of buffy coat were collected and cryoprocessing was done by automatic controlled-rate freezer. Good
recovery .of total WBC, CD34+ cells, progenitor cells BFU-E and CFU-GM after buffy coat separa
tion were observed 89 per cent, 95 per cent, 109 per cent, and 102 per cent respectively. There was no
aerobic bacterial or fungal contamination in the separated blood products. By using this technique, the
UCB units were easily collected, rapidly separated within one hour, and high recovery of the hemato
poietic progenitor cells could be obtained.
Keywords : CD34+ Cell, Stem Cell Collection, Umbilical Cord Blood
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