Wattanachai Ungjaroenwathana MD*, Chanatee Bunyaratavej MD*, Piyaratana Tosukhowong MSc**, Thasinas Dissayabutra MD**
Affiliation : * Department of Surgery, Faculty of Medicine, Chulalongkorn University, Bangkok ** Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok
Objectives : Blood loss in transurethral resection of prostate (TUR-P) operation is estimated by the difference
between pre- and post-operative hemoglobin (Hb) concentration. The authors introduced a novel practical
method to estimate blood loss in the patients who were surgically managed with TUR-P operation.
Materials and Methods : Complete blood count was collected pre-operative, immediate post-operative, and 24-
hour post-operative to determine red blood cells and Hb concentration. Hemoglobin of irrigating fluid was
measured by standard spectrophotometry and blood loss was estimated by the authors’ calculation. Irrigating
fluid was frozen and thawed to completely hemolyse the red blood cells, then it was tested by urine-strips and
calculated  for  red  cells  using  estimating  cell  ranges  given  by  the  product’s  prescription.  The  correlation
between these indicators was evaluated.
Results :  Calculated  blood  loss  detected  by  spectrophotometric  method  has  no  correlation  with  immediate
post-operative or 24-hour post-operative Hb concentration. However, it had a significant positive correlation
with calculated blood loss by urine-strip technique (r = 0.897, p = 0.01).
Conclusion : Urine-strip method can be used to estimate total blood loss in irrigating fluid in patients with
TUR-P operation. This is practical and useful in immediate post-operative evaluation of blood loss to consider
the need of blood transfusion.
Keywords : Estimation of blood loss, Transurethral resection of prostate, Urine-strip, Spectrophotometry, Hemoglobin, Irrigating fluid
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