Co-Stimulatory Molecules on Peripheral Blood Mononuclear Cells and Tissue Infiltrating Cells of Skin Wart and In Vitro Poke Weed Mitogen Stimulation
Potjaman Phumeesat MSc*, Somyot Charuwichitratana MD**, Molvibha Vongsakul PhD*
Affiliation : * Department of Microbiology, Faculty of Science, Mahidol University ** Department of Dermatology, Ramathibodi Hospital
Background : Skin wart is a lesion caused by human papilloma viruses (HPVs) that can infect both male and
female.
Objective : Quantify the number of CD28+, CD86+, CD152+ and γδ + in peripheral blood mononuclear cells
(PBMCs) of subjects with skin wart. Identify CD86+ and γδ+ cells in skin wart cryosections.
Materials and Methods : Sixteen subjects with skin warts on face, hand, finger, knee, foot or plantar, both male
and female, aged between 19-59 years-old, were recruited from Ramathibodi Hospital, Mahidol University,
Bangkok
Results : CD86 and CD152, on peripheral blood mononuclear cells (PBMCs) of subjects with skin wart are
significantly lower compared to controls. Tissue cryosection staining for CD86+ and γδ+ cells showed no
difference among subjects with skin wart and control. Proliferative response to poke weed mitogen of subjects
with skin wart is significantly lower than control subjects.
Conclusion : There was no difference in the number of subjects positive for CD28 and CD86+ cell between
normal and skin wart subject, but an increase in skin wart subjects with γδ+ cells.
Keywords : Skin wart, Co-stimulatory molecule, Immune cell competency
