Validity Evaluation of In-House Preparation Kit,
Vaginal pH Paper Test Combined Amine Tube Test,
for the Simple Diagnosis of Bacterial Vaginosis
Suchera Loyprasert-Thananimit PhD*,**, Phanida Kuasuwan (Science student)**,
Kesara Nittayaboon (Science student)**, Wilaiwan Chotigeat PhD*,**,
Nutthaporn Chandeying MD***, Verapol Chandeying MD***
* Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, Thailand
** Department of Molecular Biotechnology and Bioinformatics, Faculty of Science,
Prince of Songkla University, Hat Yai, Songkhla, Thailand
*** Department of Obstetrics and Gynecology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand
Affiliation :
Objective : Determine the positive in-house preparation kit for suggested bacterial vaginosis (BV) for both elevated vaginal
pH > 4.5 and positive amine test, as well as evaluate for validity of sensitivity, specifi city, positive predictive value, and
negative predictive value against Chandeying criteria for confi rmed BV.
Material and Method: A cross-sectional study among the women who presented with an abnormal vaginal discharge (AVD)
or asymptomatic annual cervical cytology screening was done. Each vaginal discharge was divided into two parts of
investigation. The fi rst part included the clinical criteria of confi rmed BV, based on at least three out of fi ve indicators, the
vaginal pH > 4.5, homogeneous and thin discharge (milky discharge), positive sniff/amine test, clue cell > 20% of total
vaginal epithelial cells, and scanty or absence lactobacilli. The second part included the in-house preparation kit of suggestive
BV, relied on elevated vaginal pH > 4.5 and positive amine tube test.
Results : Twenty-six women were enrolled. Of the complaint of AVD/asymptomatic had 2/10 of confi rmed BV (12 cases),
and 1/13 of confi rmed non-BV (14 cases). The in-house preparation kit, compared with the clinical criteria, had sensitivity
of 91%, specifi city of 71%, positive predictive value of 73%, and negative predictive value of 90%. There were false negative
of 1/12 cases (8.3%), and false positive of 4/14 cases (28.5%).
Conclusion : The in-house preparation kit favorably compared with the clinical criteria and has the advantage of being
simple, rapid, and easily performed in resource poor setting. Further development on sensitivity and specifi city of the test
is suggested.
Keywords : Bacterial vaginosis, In-house, pH paper test, Amine tube test
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