Conventional and Novel Methods for Embryonic Stem Cell Line Derivation
Sorapop Kiatpongsan MD*,**, Yuen Tannirandorn MD*, Pranee Numchaisrikha BSc*,**, Ruttachuk Rungsiwiwut DVM*,**
Affiliation : * Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University ** Chulalongkorn Gene and Stem Cell Therapy Research Program
Cell therapy is the promising therapeutic tool for the next decade. “Regenerative Medicine” based on cell and tissue replacement therapy is proposed as a revolutionary approach to various chronic and incurable conditions. The first key step for successful cell therapy is the establishment of clinical grade human Embryonic Stem Cell (hESC) lines. This article provides a concise summary on conventional and novel methods for hESC line derivation. There is also discussion on progression, future direction and problems in hESC line development. In Thailand, more advance knowledge, skill, and technology are required to develop the first human embryonic stem cell line and step forward to make cell therapy a reality.
Keywords : Embryonic stem cell, Derivation, Nuclear transfer, Single blastomere
