Decrease of Mosquito Salivary Gland Proteins after a Blood Meal: An Implication for Pathogenesis of Mosquito Bite Allergy
Padet Siriyasatien MD, PhD*, Kuntida Tangthongchaiwiriya MSc* Kanyarat Kraivichian MD*, Surang Nuchprayoon MD, PhD* Apiwat Tawatsin MAppl Sc**, Usavadee Thavara PhD**
Affiliation : * Department of Parasitology, Faculty of Medicine, Chulalongkorn University ** National Institute of Health, Department of Medical Sciences, Nonthaburi
Salivary gland protein profiles of Aedes aegypti (L.) and Culex quinquefasciatus (Say) pre- and post- blood feeding were analyzed. SDS-PAGE studies before blood feeding of Ae. aegypti demonstrated 8 major polypeptide bands of 20, 35, 37, 42, 45, 47, 70 kDa and a high molecular weight band >118 kDa, whereas those of Cx. quinquefasciatus demonstrated 9 major polypeptide bands of 20, 26, 36, 38, 45, 47, 49 kDa and 2 high molecular weight bands >118 kDa. After a blood feeding, salivary gland polypeptides of Ae. aegypti at 35, 37, 45, 47, 70 kDa and high molecular weight band >118 kDa were depleted, while the polypeptide bands of 20, 26, 36, 38 kDa were depleted in Cx. quinquefasciatus. The presented study suggests that these major polypeptides were introduced into vertebrate hosts when a mosquito took a blood meal. Further investigation in molecular, biochemical and immunological aspects of these salivary gland polypeptides may provide information for better understanding in the role of these proteins in mosquito bite allergy.
Keywords : Mosquito bite allergy, Aedes aegypti, Culex quinquefasciatus, Mosquito salivary gland protein
