The Distribution of IL-10 Promoter Polymorphism in Thais
Janjuree Netsawang BSc*, Marut Tangwattanachuleeporn BSc*, Nattiya Hirankarn MD, PhD**, Jongkonnee Wongpiyabovorn MD, PhD**
Affiliation : * Inter-department of Medical Microbiology, Graduate School, Faculty of Medicine, Chulalongkorn University ** Department of Microbiology, Faculty of Medicine, Chulalongkorn University
IL-10 is a regulatory cytokine, which plays important roles in the pathogenesis of many diseases Polymorphism of IL-10 promoter influences the phenotypic expressions such as the variation of IL-10 production among individuals and is subjected to the genetic susceptibility study of many diseases. However, there is no information about the frequencies of IL-10 promoter polymorphism in a Thai population. To determine the distribution of IL-10 promoter polymorphism in unrelated healthy Thais, genomic-DNA from 160 unrelated healthy volunteers were analyzed by polymerase chain reac- tion-restriction fragment length polymorphism (PCR-RFLP) method. The functional single-nucleotide polymorphisms (SNPs) in IL-10 promoter (positions -1082 (G/A), -819 (C/T), -592 (C/A) were included. The allele frequencies of -1082*A (93.4%), -819*T (71.9%), and -592*A (71.9%) were significantly higher than the allele frequencies of -1082*G (6.6%), - 819*C (28.1%), and -592*C (28.1%) respectively in a Thai population similar to other Asian populations (Korean, Japanese, Chinese). As for the haplotype analysis, the ATA haplotype (72%) was significantly higher in Thais and other Asian populations compared to non-Asian populations; whereas, GCC haplotype (6.6%) was significantly lower in Thais. Additionally, two rare haplotypes of IL-10 promoter (ATC and ACA) which were previously reported only in the Chinese Han people, were found with similar frequencies (0.6%) in the present study. In conclusion, the distribution of IL-10 promoter polymorphisms in Thais was comparable to other Asian populations but distinct from Non-Asian populations. At least five haplotypes existed in an unrelated healthy Thai population as ACC, GCC, ATA, ATC, and ACA haplotypes.
Keywords : IL-10, Single-nucleotide polymorphisms
