Component of Manual Preparation of Platelet-Rich Plasma
Chalermsak Sriworakun MD*, Thanarat Reancharoen MD**, Cholawish Chanlalit MD**
Affiliation : * Department of Orthopedics, Faculty of Medicine, Vajira Hospital, Bangkok, Thailand ** Department of Orthopedics, Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand
Background : Popularity of using Platelets Rich Plasma (PRP) in the orthopedics practice is increasing, however, commercial
kits for preparation are typically expensive.
Objective : To clarify the best protocols of manual PRP preparation that can obtain the highest platelet concentration with low
amount of white blood cells and red blood cell.
Material and Method: A 22 ml blood samples was taken from 10 healthy volunteers. Each specimen was equally separated
into 4 syringes for 4 different protocols of centrifugation duration; 1,000 rpm/2 mins, 1,000 rpm/3 mins, 1,000 rpm/5 mins
and 1,000 rpm/7 mins. Platelet counts from each protocol was analyzed.
Results : The protocol of 2 and 3 minutes spinning duration have shown higher platelets concentration compared to other
protocols. The highest amount of platelet concentration was obtained from the 3-minute duration protocol at a mean of
438.1+124.733x103/mm3 (1.7 fold than baseline) and also lower WBC contamination when compared to 2-minute duration
protocol.
Conclusion : The spinning of blood at 1,000 rpm for 3 minutes is the best protocol to gain plasma that has a high platelet
concentration and low WBC contamination.
Keywords : Platelet-rich plasma, Manual PRP preparation, Platelet concentration
