Andrographolide Ameliorates Beta-Naphthoflavone- Induced CYP1A Enzyme Activity and Lipid Peroxidation in Hamsters with Acute Opisthorchiasis
Latiporn Udomsuk PhD*,**, Waranya Chatuphonprasert PhD**,***, Kanokwan Jarukamjorn PhD**, Paiboon Sithithaworn PhD****
Affiliation : * College of Medicine and Public Health, Ubon Ratchatani Universty, Ubon Ratchatani, Thailand ** Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand *** Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand **** Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand
Background : Opisthorchis viverrini (OV) infection generates oxidative stress/free radicals and is considered as a primary
cause of cholangiocarcinoma since it primarily triggers sclerosing cholangitis.
Objective : In this study, the impacts of andrographolide on acute opisthorchaisis in β-naphthoflavone (BNF)-exposed
hamsters were investigated.
Material and Method: Ethoxyresorufin O-deethylase (EROD) and methoxyresorufin O-demethylase (MROD) activities and
Thiobarbituric acid reaction substances (TBARS) assay of andrographolide in acute opisthorchiasis in the BNF-exposed
hamsters were assessed.
Results : The results showed that andrographolide ameliorated the hepatic CYP1A1 and CYP1A2 activities by decreases of the
specific enzymatic reactions of EROD and MROD, respectively, in the BNF-exposed hamsters. Moreover, andrographolide
lowered the formation of malondialdehyde in the livers and brains of the hamsters.
Conclusion : These observations revealed the promising chemo-protective and antioxidant activities of andrographolide via
suppression of the specific EROD and MROD reactions and lipid peroxidation against acute opisthorchiasis in the BNF-
exposed hamsters.
Keywords : Opisthorchiasis, Andrographolide, β-naphthoflavone, CYP1A, Lipid peroxidation, Hamster
