Antimicrobial Activity of Thai Medicinal Preparation of Khampramong Temple Used for Cancer Treatment and Its Plant Components
Onmanee Prajuabjinda MSc*, Sumalee Panthong BSc**, Arunporn Itharat PhD**
Affiliation : * Student of Master Degree on Medical Science Program, Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani, Thailand ** Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani, Thailand
Background : Thai medicinal preparation of Khampramong Temple has been used for cancer treatment more than ten years
ago. It is composed of eleven herbs. Many anticancer drugs exhibited antimicrobial activity as antitumor antibiotics such as
the anthracycline group [daunorubicin] and quinone group [mitomycin C].
Objective : To determine antimicrobial activity of Thai medicinal plants used to treat cancer patients of Khampramong Temple
by disc diffusion and agar dilution methods.
Material and Method: The extraction procedure was maceration method using 95% ethanol and drying by evaporator. In
the preliminary study, all extracts were evaluated for antimicrobial activity by disc diffusion method against two strains of
Gram positive bacteria (Staphylococus aureus and Bacillus subtilis), one Gram negative bacteria (Escherichia coli) and one
fungus (Candida albicans). The active plant extracts were diluted to determine the minimum inhibitory concentration (MIC)
by agar dilution method.
Results : The preparation showed antimicrobial activity against S. aureus, B. subtilis and E. coli (MIC = 1.25, 0.625 and
5 mg/ml, respectively) but no inhibition against Candida albicans. Most extracts showed activity against B. subtilis and
Rhinacanthus nasutus extract showed the highest antimicrobial activity (MIC = 0.156 mg/ml). Hydnophytum formicarum
Jack, Tectona grandis L.f. and Salacia chinensis L. exhibited good antibacterial activity against S. aureus (MIC = 1.25,
0.15625 and 0.3125 mg/ml respectively).
Conclusion : These results supported the use of this preparation on chronic wound infection of cancer patients and the
antimicrobial compounds of the preparation should be further studied to be used in cancer patients.
Keywords : Thai medicinal preparation, Khampramong Temple, Antimicrobial
