Comparison of Antimicrobial, Antioxidant Activities and Total Phenolic Content of Antidesma Thwaitesianum Fruit Extracts by Different Methods
Bhanuz Dechayont MSc*, Pintusorn Hansakul PhD**, Arunporn Itharat PhD*
Affiliation : * Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Klongluang, Pathumthani, Thailand ** Department of Preclinical Science, Faculty of Medicine, Thammasat University, Klongluang, Pathumthani, Thailand
Background : Antidesma thwaitesianum Mull.Arg. is a tropical fruit in Northeast Thailand and its fruits are used for soft
drink and healthy food. The biological activities of extracts from Antidesma thwaitesianum using different extraction methods
have not been reported.
Objective : Extracts from Antidesma thwaitesianum using different extraction methods were tested for antibacterial and
antioxidant activities as well as were determined for total phenolic content. Ten extracts were tested for antimicrobial activity
by disc diffusion, MIC, and MBC methods. DPPH assay was used to test antioxidant activity and Folin-Ciocalteu’s reagent
was used to determine total phenolic content.
Results : The extract obtained by decocting residue after maceration process of dried marc (MRW) exhibited stronger
antioxidative power (EC50 = 11.73 μg/ml) than BHT (EC50 = 13.36 μg/ml). This antioxidant activity was related to total
phenolic content of 85.77 + 0.34 mg GAE/g. This extract also exhibited antimicrobial activity against Staphylococcus aureus
(inhibition zone = 8 mm, MIC = 2.5 mg/ml). Moreover, the extract obtained by macerating dried marc (MME) exhibited
antimicrobial activity against Bacillus subtilis with the same MIC value of 10 mg/ml.
Conclusion : All of the extracts of Antidesma thwaitesianum had less potential for antimicrobial activity than Gentamicin and
Amphotericin B. On the other hand, the water extract especially obtained by decocting residue after maceration of dry marc
had good antioxidant power and the highest total phenolic content. Thus, such water extract should be recommended for a
good source of natural antioxidants for commercial uses.
Keywords : Antidesma thwaitesianum Mull.Arg., Antimicrobial, Antioxidant, DPPH, Total phenolic contents
