Antioxidant and Anti-inflammatory Activities of Thai Medicinal Plants in Sahasthara Remedy for Muscle Pain Treatment
Narin Kakatum MSc*, Nuanjan Jaiarree PhD** Sunita Makchucit MSc**, Arunporn Itharat PhD**
Affiliation : * Graduate School, Faculty of Medicine, Thammasat University, Pathumthani, Thailand ** Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathumthani, Thailand
Objective : Twenty one plants as ingredients of Thai traditional preparation called Sahasthara for relieve muscles pain and
distal numbness were studied for their antioxidant and anti-inflammatory activities.
Material and Method: The extracts were obtained by maceration with 95% ethanol. They were tested for their antioxidant
activity by DPPH scavenging assay and anti-inflammatory activity by determination of inhibitory activity on lipopolysaccharide
(LPS) induced nitric oxide (NO) production in RAW 264.7 cell lines using Griess reagent.
Results : The ethanolic extract of Terminalia chebula Retz. (Gall) showed the highest antioxidant activity (EC50 = 3.34 μg/ml).
Its fruit extract also exhibited the most potent inhibitory activity on lipopolysaccharide (LPS) induced nitric oxide (NO)
production in RAW 264.7 cells, followed by Atractylodes lancea and Baliospermum montanum (IC50 = 3.3, 9.7 and 12.6 μg/
ml, respectively).
Conclusion : The results obtained for anti-inflammatory and antioxidant activities of these plants correspond with their
traditional use for muscle pain and inflammatory-related diseases.
Keywords : Antioxidant, Nitric oxide inhibition assay, Anti-inflammation, Muscle pain, Sahasthara
