Different Professional Skill to Produce Platelet Rich Plasma May Effect the Clinical Results
Reancharoen T, MD1, Sriworakun C, MD2, Chanlalit C, MD1
Affiliation : 1 Department of Orthopaedics, HRH Princess Maha Chakri Sirindhorn Medical Center, Faculty of Medicine, Srinakharinwirot University, Nakhon Nayok, Thailand 2 Department of Orthopaedics, Faculty of Medicine, Vajira Hospital, Bangkok, Thailand
Background : Previous study of a Manual PRP preparation which stated clearly about the procedure of producing PRP that has
platelet concentration higher than baseline. Since there are steps that require skills of performers that prepare the manual PRP,
there should be a study of probability that the Manual PRP preparation can minimize human error.
Objective : To study the reproducibility of the PRP produced by the manual PRP preparation. The quality of PRP from manual PRP
preparation performed by doctor and nurse, including platelet concentration with low levels of white and red blood cells, will be
compared.
Materials and Methods : In the present study, the performers in this study were the orthopedic resident and the nurse who worked
in the orthopaedic out-patient department at HRH Princess Maha Chakri Sirindhorn Medical Center as the representatives of
different professions. 12 volunteers were participated. The samples of PRP obtained were then given to the laboratory for analysis
to obtain platelet concentration.
Results : The PRP performed by doctor had a higher concentration of platelet and lower concentration of WBCs when compared
with PRP performed by the nurse. The difference in platelet concentration of PRP from manual PRP preparation between samples
performed by doctor and nurse was significant. The difference between the WBC count of PRP from manual PRP preparation
carried out by doctor and nurse was significant but the difference of RBC count was not significant.
Conclusion : The different medical profession of the performers, which doctors and nurses were the representatives in the present
study, gives a statistically significant difference in platelet concentration and the amount of WBCs and RBC.
Keywords : Platelet-rich plasma, PRP, Manual PRP preparation, Platelet concentration, Professional skill
