The Inhibitory Effect of 13-Butoxyberberine Bromide on Migration in Breast Cancer MDA-MB-231 Cells
Laomethakorn P, BSc1, Jaitrong M, MSc2, Samosorn S, PhD2, Watanapokasin R, PhD1
Affiliation : 1 Department of Biochemistry, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand 2 Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok, Thailand
Background : 13-butoxyberberine bromide is a berberine derivative that has not been reported on the anti-migration mechanism
of breast cancer. Cancer metastasis is the major cause of cancer mortality, about 90% of cancer patients death are caused by cancer
metastasis not the primary tumor. Therefore, the compound that could inhibit cancer migration is necessary for development as
anti-cancer drug.
Objective : To investigate the effects of 13-butoxyberberine bromide on the anti-migration of breast cancer MDA-MB-231 cells.
Materials and Methods : Cell viability was determined by MTT assay. Wound-healing assay and transwell chamber assay were used
to determine cell migration. Protein expression levels were examined by western blot analysis.
Results : 13-Butoxyberberine bromide at sub-toxic concentration (10 μg/mL) inhibited cell migration in MDA-MB-231 treated
cells via down-regulation of MMP-2 and MMP-9 expression which play important role in cancer cell metastasis.
Conclusion : 13-Butoxyberberine bromide at 10 μg/mL showed anti-migration effect against MDA-MB-231 cells through inhibition
of mediator proteins. These findings could be useful in the development of 13-butoxyberberine bromide to be a novel anti-migration
agent for clinical use in the future.
Keywords : Breast cancer, 13-butoxyberberine bromide, Migration, MMPs
