Retrospective Karyotype Analysis of 12,261 Cases with Hematological Malignancy
Phanasit Phuwasanpetch MSc1, Thivaratana Sinthuwiwat MSc1,2, Jutamas Yimnoon MSc1, Chirayu Auewarakul MD, PhD2,3, Priyavudh Herabutya PhD1,2
Affiliation : 1 Cancer Cytogenetics Unit, Division of Research and International Relations, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal Academy, Bangkok, Thailand 2 Faculty of Medicine and Public Health, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal Academy, Bangkok, Thailand 3 Department of Medicine, Faculty of Medicine Siriraj Hospital, Bangkok, Thailand
Objective : To report characteristics and number of chromosome abnormalities detected in samples collected from patients
suspected of having haematological malignancy by clinicians.
Materials and Methods : We retrospectively reviewed karyotype data of 12,261 newly diagnosed haematological malignancy
and follow-up cases who received conventional cytogenetic analyses at Cancer Cytogenetics Unit, Chulabhorn Hospital
between 2009 and 2015.
Results : Among the total suspected and follow-up haematological malignancy cases analysed, 35.22% (4,318 cases) had
chromosomal aberrations while the other 64.78% (7,943 cases) showed normal chromosome arrangements. Among the
patients with chromosomal aberrations, there were 734 cases with numerical aberrations, another 2,621 cases with structural
aberrations, and a further 963 cases reported with both numerical and structural aberrations. Moreover, we discovered in
Thai chronic myelogenous leukaemia [CML] patients with complex translocations that a third and fourth chromosome were
sometimes involved with the Philadelphia chromosome (9; 22) (q34; q11), creating three-way and four-way translocations.
Conclusion : The present study compiles the karyotypic analysis of 12,261 haematological cases which represents the
largest dataset from Thailand. Chromosomal aberrations were found in one-third of total cases. Our information should be of
value to include in a national cancer registry.
Keywords : chromosome, haematological malignancy, karyotype, conventional cytogenetics, chromosomal aberrations
