DNA Methylation Assessment in Cervical Cancer Specimens
Wandee Udomchaiprasertkul MSc1, Nuttavut Kantathavorn MD2,3, Thaniya Sricharunrat MD4, Kanyanee Laebua MD5, Pornwaree Trirussapanich MD5, Chakrit Bunyoo MSc1, Kriangpol Wiriyaukaradecha MSc1, Narongrit Sritana MSc1, Sasikarn Chamchod MD3,5, Chirayu Auewarakul MD, PhD3
Affiliation : 1 Molecular Biology and Genomic Research Laboratory, Division of Research and International Relations, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal Academy, Bangkok, Thailand 2 Gynecologic Oncology Unit, Woman Health Center, Chulabhorn Hospital, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal Academy, Bangkok, Thailand 3 Faculty of Medicine and Public Health, Chulabhorn Hospital, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal Academy, Bangkok, Thailand 4 Pathology Laboratory Unit, Chulabhorn Hospital, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal Academy, Bangkok, Thailand 5 Department of Radiation Oncology, Chulabhorn Hospital, HRH Princess Chulabhorn College of Medical Science, Chulabhorn Royal Academy, Bangkok, Thailand
Objective : To determine methylation levels of DAPK1, MGMT, FAS, TRAIL-R1 and p73 genes in formalin fixed, paraffin
embedded [FFPE] tissues from patients with cervical cancer stage I-IV using high resolution melting [HRM].
Materials and Methods: DNA was extracted from FFPE of 63 cervical cancer specimens and 42 normal specimens.
Methylation levels of DAPK1, MGMT, FAS, TRAIL-R1 and p73 genes were detected by HRM.
Results : The methylation levels of DAPK1, FAS, TRAIL-R1 and p73 genes showed statistically significant differences
between the disease and the control cases (p<0.005). In contrast, the methylation of MGMT showed no significant
difference between the disease and the control cases. DAPK1 methylation level was associated with squamous cell carcinoma
patients.
Conclusion : DAPK1, FAS, TRAIL-R1 and p73 are aberrantly methylated in cervical cancer compared with normal tissue,
while MGMT is less commonly methylated. HRM can be used for DNA methylation assessment of genes of interest in
cervical cancer FFPE specimens.
Keywords : Cervical cancer, DNA methylation, High resolution melting [HRM], Epigenetics, Disease markers
