A Randomized Trial of Partial Versus Complete Versus Not Using Laser-Assisted Hatching in Vitrified-Warmed Cleavage Embryo Transfer: A Preliminary Report

Usanee Sanmee MD¹, Waraporn Piromlertamorn BSc¹, Teraporn Vutyavanich MD¹

Affiliation : ¹ Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

Background : Currently, the effect of laser-assisted hatching (LAH) on the outcome of cryopreserved embryo remains controversial and unclear, especially on the cryopreserved embryos using a novel vitrification method.
Objective : To compare the pregnancy outcomes of vitrified-warmed cleavage stage embryos transfer using LAH breaching or LAH thinning versus those not using LAH.
Materials and Methods : Sixty patients with vitrified-warmed cleavage embryo transfer were randomly assigned to a control group without LAH treatment, LAH-breeching group, and LAH-thinning group. The outcome measurements were clinical pregnancy rate, implantation rate, and live birth rate.
Results : The clinical pregnancy rate (35% versus 20% versus 25%) and implantation rate (17.3% versus 11.5% versus 11.3%) were lower in both LAH-breaching and LAH-thinning group than the control group, but not statistically significant (p>0.05). The live birth rate (30% versus 5% versus 5%) was significantly lower in both the LAH-breaching and LAH-thinning group than the control group (p=0.026).
Conclusion : LAH regardless of breaching or thinning methods significantly decreases live birth rate in vitrified-warmed cleavage-stage embryo transfer.

Received 8 June 2020 | Revised 6 August 2020 | Accepted 6 August 2020
doi.org/10.35755/jmedassocthai.2021.01.11515

Keywords : Laser-assisted hatching, Vitrified-warmed, Cleavage embryo

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