Acyclovir Susceptibility of Herpes Simplex Virus Isolates at King Chulalongkorn Memorial Hospital, Bangkok

Ajchariyarat Sangdara MSc*, Parvapan Bhattarakosol PhD**

Affiliation : * Inter-department of Medical Microbiology, Faculty of Graduate School, Chulalongkorn University, Bangkok ** Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok

Objective : To determine the ACV susceptibility in Thai HSV clinical isolates. Material and Method: One hundred thirty HSV isolates from the Virology Laboratory Unit, King Chulalongkorn Memorial Hospital, Bangkok, Thailand had typing done by immunofluoresent assay using monoclonal antibody specific to either HSV-1 or HSV-2. Their sensitivity to ACV (IC50) was determined by plaque reduction assay.
Results : The IC50 of 77 HSV-1 isolates ranged from 0.07-0.97 μg/ml and that of 53 HSV-2 isolates was 0.13-1.66 μg/ml. The standard HSV-1 (KOS) and HSV-2 (Baylor 186) were included in each run. The mean + standard deviation (SD) of ACV IC50 among HSV-1 and HSV-2 isolates were 0.38 + 0.23 and 0.50 + 0.32 μg/ml while that of standard HSV-1 and HSV-2 were 0.45 + 0.13 and 0.57 + 0.04 μg/ml. Statistically significant difference between IC50 of HSV-1 and HSV-2 isolates was indicated (p = 0.02).
Conclusion : No ACVr HSV has been detected and ACV susceptibility of HSV-2 has more resistance than that of HSV-1.

Keywords : HSV, Acyclovir susceptibility, King Chulalongkorn Memorial Hospital

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