Paraoxonase 1 (PON1) L55M and Q192R Polymorphisms
are Associated with Type 2 Diabetes Mellitus in Southern
Thai Subjects
Didas N, MSc¹, Thitisopee W, MD², Porntadavity S, PhD³, Nuinoon M, PhD¹, Bhoopong P, PhD¹, Jeenduang N, PhD¹
Affiliation : ¹ School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, Thailand ² Department of Medicine, Thasala Hospital, Nakhon Si Thammarat, Thailand ³ Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Bangkok, Thailand
Background: Paraoxonase 1 (PON1) plays a critical role in the prevention of cardiovascular disease, diabetes mellitus, and
other chronic diseases.
Objective: The aim of the present study was to investigate the association of PON1 L55M and Q192R polymorphisms with type 2 diabetes mellitus (T2DM), and pre-diabetes.
Materials and Methods: The present study included 512 subjects (223 T2DM patients, 150 pre-diabetes, and 139 healthy controls) from Southern Thailand. The PON1 L55M and Q192R polymorphisms were analyzed by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) technique.
Results: PON1 Q192R polymorphism showed a statistical difference in genotype frequencies between T2DM patients and healthy controls (p<0.0001). Multiple logistic regression analyses after adjusting for age, gender, and BMI showed that LM and RR genotypes increased the risk for T2DM compared with LL and QQ+QR genotypes (OR 1.96; 95% CI 1.02 to 3.76, p=0.042, and OR 1.85; 95% CI 1.11 to 3.10, p=0.019, respectively). Whereas, QR, genotype was associated with decreased risk for T2DM (OR 0.240; 95% CI 0.13 to 0.45, p<0.0001), and pre-diabetes (OR 0.549; 95% CI 0.30 to 0.99, p=0.048) compared with QQ genotype.
Conclusion: PON1 LM and RR genotypes may be genetic risk factors for developing T2DM but QR genotype may prevent T2DM, and pre-diabetes in Southern Thai population.
Received 14 Jan 2020 | Revised 4 Mar 2020 | Accepted 6 Mar 2020
Keywords : PON1, Polymorphisms, Type 2 diabetes, Pre-diabetes
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