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Impact of SLC47A1 (rs2289669 G>A) Variant on Metformin Associated Lactic Acidosis Patients

Pechngam Chaivanit MD1, Somchai Yongsiri MD1, Pakaphan Dinchuthai MD1, Objoon Trachoo MD2

Affiliation : 1 Department of medicine, Faculty of Medicine, Burapha University, Chonburi, Thailand 2 Department of medicine, Faculty of Medicine, Mahidol University, Bangkok, Thailand


Objective : To address the important role of SLC47A1 G>A (rs2289669) variants in type 2 diabetic Thai patients. The present study was undertaken to investigate the association between disease severity and incidence of those type 2 diabetic patients harboring this genetic abnormality.
Materials and Methods : Between January 2014 and December 2015, all patients diagnosed with metformin associated lactic acidosis [MALA] in Burapha University Hospital were enrolled. Full medical record and treatment outcome were collected. RT-PCR were performed in blood samples to identify the SLC47A1 G>A (rs2289669) variations genotyping.
Results : There were 10 patients (age 69.4±12.6 years, duration of diabetes 12.1±3.51 years, metformin dose 2,000±471.4 mg/day). The incidence of AA-alleles in MALA patients was 50%, which was signi(cid:976)icantly higher than the general type 2 diabetic patients (p<0.0001, 95% CI 3.09 to 8.01). Serum creatinine and anion gap of AA patients were signi(cid:976)icantly higher than non-AA alleles, (7.79+1.80 vs. 3.80+1.77 mg/dL, p = 0.032), (34.2+4.66 vs. 22.0+8.25 mEq/L, p = 0.045), respectively.
Conclusion : The present study has been demonstrated the incidence of homozygous MATE/SLC47 gene polymorphism in MALA patients was higher than general type 2 diabetic patient. MALA patients who carry this genetic mutation have more severe disease than those who do not carry this mutation.

Keywords : MATE1, SLC47A1, Metformin associate lactic acidosis, DM type 2


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