Occurrence of P392L Mutation in Ethnic Thai with Chinese
Descent Paget’s Disease
Vorachai Sirikulchayanonta MD1, Anchalee Tantiwetrueangdet MSc2
Affiliation :
1 Faculty of Science, Rangsit University, Pathum Thani, Thailand
2 Research Center, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Background : The etiology of Paget’s disease is still unclear. However, the available evidence shows that genetics plays an important
role in its pathogenesis. Although the disease is genetically heterogeneous, mutations of SQSTM1 gene, especially missense mutation
P392L, are reportedly the most common genetic polymorphism associated with it. To the best of our knowledge, data regarding
mutations of this gene have mainly been obtained from patients of European descent. The authors hypothesized that the P392L
mutation might also play a role in Paget’s disease among Asian ethnic groups. Unfortunately, due to the rarity of the cases, data
regarding mutations among Asian ethnic groups, especially those from Southeast Asian countries are sparse in the literature.
Objective : To (cid:976)ind the occurrence of P392L mutation in Paget’s disease among ethnic Thai group.
Materials and Methods : A genetic analysis was carried out using archived material from four ethnic Thai patients with Paget’s
disease. DNA was extracted from formalin-(cid:976)ixed, paraf(cid:976)in embedded tissue for speci(cid:976)ic PCR targeting of 326 bp of the SQSTM1 gene
(exon 8). Amplicons were sequenced bidirectionally using an ABI PRISM 3730XL (Applied Biosystems, USA) sequencer using T7
promoter and SP6 primers.
Results : No P393L mutations in the SQSTM1 protein (exon 8) were found in any of these four cases.
Conclusion : These results were similar to those obtained from previous studies on patients of Chinese descent. A combined analysis
of the present data and that from 21 previously-reported, sporadic cases of Paget’s disease in Asian patients revealed absence of
the P392L mutation but presence of one different mutation (1/25 = 4%).
Keywords : Sequestosome 1, Paget’s disease, SQSTM1, Ethnic Thai, P392L, Chinese
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