Human Papillomavirus DNA in Paraf(cid:976)in-Embedded
Retinoblastoma
Jarin Saktanasate MD1, Piangporn Saksiriwutto MD1, Mongkol Uiprasertkul MD2,
Navin Horthongkham MD3, Adisak Trinavarat MD1, La-ongsri Atchaneeyasakul MD1
Affiliation :
1 Department of Ophthalmology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
2 Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
3 Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
Background : Human papillomavirus [HPV] has been shown to cause several cancers including cervical, anal, and oropharyngeal
by interfering with the tumor suppressor protein function resulting in unregulated cell proliferation. The virus can replicate only
in strati(cid:976)ied epithelium by direct contact to the basal cell layer. For retinoblastoma, the most common primary intraocular malignancy
in children, the tumor derives from immature retinal cells originating from the neuroepithelium. Controversy remains regarding
the detection of HPV-DNA in retinoblastoma.
Objective : To investigate the presence of HPV genomic sequence in retinoblastoma tissues.
Materials and Methods : One hundred seventeen paraf(cid:976)in-embedded retinoblastoma samples between January 2000 and December
2013 were included in the study. Real-time polymerase chain reaction was used to identify HPV genomic sequence from the samples.
We used 21 paraf(cid:976)in-embedded ocular tissues, which enucleated from other causes as a control group. We used the same tissue
preparation and process in both groups.
Results : After removing 37 (31.6%) invalid data from 117 retinoblastoma samples, we did not identify HPV-DNA in any of 80 (68.4%)
valid data samples. In control group, HPV-DNA was not found in 12 (57.1%) valid data samples. Nine (42.9%) samples were invalid.
Conclusion : Our results con(cid:976)irm the negative correlation between the HPV and retinoblastoma, which can be explained by the
virulence mechanisms of this oncogenic virus.
Keywords : Human papillomavirus, Retinoblastoma, Oncogenic virus, Polymerase chain reaction, Health promotion and prevention
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