The Effect of Different Timing after Ejaculation on
Sperm Motility and Viability in Semen Analysis at
Room Temperature
Sirisuk Ouitrakul MD1, Matchuporn Sukprasert MD1, Chatchai Treetampinich MD1, Wicharn Choktanasiri MD1,
Sakda Arj-Ong Vallibhakara MD, PhD2, Chonthicha Satirapod MD1
Affiliation :
1 Infertility Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
2 Section for Clinical Epidemiology and Biostatistics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Objective : To determine the time variated effect on the motility, viability, and morphology parameters of sperm after ejaculation
(30, 60, 120, 180, and 240 minutes).
Materials and Methods : The present study was an observational study that included 98 semen samples. Semen samples were kept
in room temperature (25°C to 26°C) and were analyzed for sperm motility by computer aided sperm analysis [CASA] system and
sperm viability by eosin test at 30, 60,120, 180, and 240 minutes and sperm morphology by Diff-Quick rapid test at 30 and 180
minutes.
Results : Ninety-eight semen samples were collected from 70 men. Mean age was 34.6±6.6 years old, the average BMI was 24.1±2.9
kg/m2. Mean of sperm motility at 30, 60, 120, 180, and 240 minutes were 64.14±14.40%, 59.57±16.44%, 57.46±16.50%,
54.63±18.18%, and 54.60±18.52%, respectively. Sperm motility signi(cid:976)icantly decreased after keeping in room temperature for 60
minutes after ejaculation (p-value <0.04). Mean of sperm viability at 30, 60, 120, 180, and 240 minutes were 67.59±13.20%,
65.88±12.33%, 62.10±11%, 58.60±14.08%, and 54.10±10.37%, respectively. Sperm viability signi(cid:976)icantly decreased after keeping
in room temperature for 120 minutes after ejaculation (p-value <0.001).
Conclusion : The motion parameters of sperm begin to decline in movement after 60 minutes, whereas velocity, and viability of
sperm is affected after 120 minutes. Therefore, for effective and accurate semen analyses, the semen samples should be tested
within one hour after ejaculation and should be stored at room temperature.
Keywords : Sperm motility, Sperm viability, Sperm velocity, Semen analysis, Time variated analysis
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