Application of Gelatin-Coated Magnetic Particles for Isolation of Genomic DNA from Bones
Pongsak Khanpetch BSc*, Sorasak Intorasoot PhD**, Sukon Prasitwattanseree PhD***, Karnda Mekjaidee MD****, Pasuk Mahakkanukrauh MD*,*****
Affiliation : * Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand ** Division of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand *** Department of Statistics, Faculty of Science , Chiang Mai University, Chiang Mai, Thailand **** Department of Forensic Medicine, Chiang Mai University, Chiang Mai, Thailand ***** Excellence Center in Osteology Research and Training Center (ORTC), Chiang Mai University, Chiang Mai, Thailand
Objective : To develop a method for human genomic DNA extraction from bone using gelatin-coated magnetic particles.
Material and Method: Thirty human metacarpal with the bone age ranging from 36 to 93 years were included in the present
study. Genomic DNA was extracted from bones using gelatin-coated magnetic particles. The concentration and purity of
DNA were analyzed in comparison with a reference method. In addition, the quality of extracted DNA was examined for
sex determination by conventional polymerase chain reaction (PCR).
Results : The average DNA concentration using gelatin coated magnetic particles exhibited approximately 15 times higher
than a reference method with an insignificantly difference of the DNA purity in both methods. Twelve (40%) and fifteen
(50%) samples out of thirty DNA isolated using established and reference method, respectively, could be amplified and sex
correctly determined by PCR.
Conclusion : Gelatin coated magnetic particle is rapid, simple, and well-suited for isolation of DNA from bones.
Keywords : Gelatin-coated magnetic particles, Sex determination, polymerase chain reaction (PCR)
