Control Cells for Both Electrical Impedance and Light Scattering Automated Hematological Analyzers: Pre- paration from Normal and Thalassemic Blood Samples
PASRA ARNUTTI, M.Sc. *, KOVIT PATANAPANYASAT, Ph.D.***, AHNOND BUNYARATAVEJ, Ph.D.**, AMPAIWAN CHUANSUMRIT,M.D.****
Affiliation : *Hematology Division, Department of Pediatrics, Pramongkutklao Hospital, Bangkok 10400, **Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, ***Office for Research and Development, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, ****Department of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
Abstract The study on quality control of automated blood cell analyzers, Technicon H* 1 and Coulter MAXM by using three separately self-prepared control cells was extensively investigated. The three parts of control cells are pseudo-leukocyte and fixed platelets, which are fixed by gluta- raldehyde, and control red cells from normal and thalassemic patients preserved and antico- agulated in CPO or CPDA-1. The Technicon H*l system was based on the principle of light scattering but the Coulter MAXM was based on the principle of electrical impedance for cell counting and measurement. The self-prepared control cells can be satisfactorily utilized as control for each system with statistically significant difference (p < 0.05) for both systems. The expirated dates for control cells are different in both systems and should be determined for each system spe- cifically. The control red cells prepared from thalassemic patients were quite satisfactorily useful as an abnormal control for both systems during this study.
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